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Research
3.
Identification of biovars of Brucella abortus
in aborted cattle and buffaloes herd in Sri
Lanka -
M. A. R. Priyantha
Vet World. 2011; 4(12): 542-545
doi: 10.5455/vetworld.2011.542-545
Abstract
Bovine brucellosis is an endemic disease in Sri
Lanka, caused by Brucella abortus and had been
reported all part of the country for last six
decades. Since available biovar is still unknown,
the objective of the study was to identify the
biovar of B.abortus from sporadically aborted
cattle and buffaloes. Samples were collected from
18 aborted herds out of 19 herds of Cattle and
Buffaloes in the year 2010. Rose Bengal plate test
and Compliment Fixation test were carried out.
Milk, vaginal swabs, placental contents and
aborted fetus were collected and cultured by
conventional bacteriological methods. The
detection of biovars were based on growth on
Thionin and Bacto fuschin,CO2 requirement, H2S
production, serum agglutination with Brucella
negative, A,M and R reference antiserum. Eighteen
herds investigated out of 19 herds reported, 11
herds were serologically positive for brucellosis
(61.11%) and only Brucella abortus were isolated
from 8 individuals from six herds. All were
identified as Biovar 3 of Brucella abortus.
Keywords: Bovine, Brucellosis, Sri Lanka,
Endemic disease, Occupational health Hazard,
Zoonoses, Laboratory Techniques, Biovars.
